Description
Bacterial Medium: LB Broth is a pre-formulated Luria-Bertani bacterial growth medium in powder form, optimized for consistent bacterial culture in molecular biology research. At 20g per liter, a 500g package prepares 25 liters of complete medium, it is ideal for labs running regular bacterial cultures, protein expression, or plasmid preparation workflows.
Technical Specifications:
- Format: Powder
- Package size: 500g
- Standard concentration: 20g/L
- pH: 7.0 ± 0.2 at 25°C
- Autoclave compatible
Composition per Liter:
- Tryptone: 10g
- Yeast Extract: 5g
- Sodium Chloride: 10g
Preparation Protocol:
1. Weigh 20g powder per liter
2. Add appropriate volume of water
3. Mix until dissolved
4. Autoclave at 121°C for 15 minutes
5. Cool to room temperature before use
Research Applications:
- Bacterial culture
- Protein expression
- Plasmid preparation
- Competent cell preparation
- Molecular biology studies
- Microbiology research
Storage Requirements:
- Store powder at room temperature
- Keep container tightly sealed
- Protect from moisture
- Prepared medium stable at 4°C
