Description
HotStart Taq DNA Polymerase
A chemically modified Taq DNA polymerase engineered with heat-activation properties, requiring initial 94°C incubation for enzyme activation. This temperature-dependent activation helps minimize non-specific amplification during PCR setup.
Technical Specifications:
- Activation temperature: 94°C
- Template-dependent polymerization
- Terminal transferase activity: 3' A overhang
- Chemical modification for heat activation
- Storage stable at -20°C
Enzyme Properties:
- Heat-activated DNA synthesis
- Temperature-dependent activity
- 5'→3' polymerase activity
- Compatible with standard PCR conditions
- Inactive at room temperature
Research Applications:
- Genomic DNA amplification
- cDNA target amplification
- Low copy number templates
- GC-rich template amplification
- Multiplex PCR protocols
- Standard PCR procedures
Protocol Benefits:
- Room temperature reaction setup
- Reduced primer-dimer formation
- Minimized non-specific amplification
- Standard PCR cycling conditions
- Compatible with various template types
Keywords: hot start PCR, thermostable DNA polymerase, PCR optimization, DNA amplification, molecular biology reagents
