Description
DNA SafeStain is a safe, non-mutagenic nucleic acid staining reagent for visualizing DNA and RNA in agarose gels. Unlike ethidium bromide, DNA SafeStain has tested negative in the Ames assay and multiple mammalian cell safety assays, making it a safer choice for routine gel documentation without compromising sensitivity.
A nucleic acid staining reagent designed for visualization of DNA and RNA in agarose gels. This stain has been tested for safety through Ames testing and mammalian cell assays.
Technical Specifications:
- Concentration: 10,000X
- Excitation peaks: 290nm, 490nm
- Emission wavelength: 530nm
- Fluorescence: Green
- Compatible with UV transilluminators
Safety Testing:
- Ames test: Negative
- Mouse marrow chromophilous erythrocyte micronucleus test: Negative
- Mouse spermary spermatocyte chromosomal aberration test: Negative
Nucleic Acid Detection:
- Double-stranded DNA
- Single-stranded DNA
- RNA molecules
- Agarose gel compatible
- Standard gel documentation systems compatible
Protocol Features:
- Direct gel staining capability
- Standard electrophoresis protocols
- Room temperature handling
- Standard UV visualization
- Compatible with typical loading buffers
Research Applications:
- DNA fragment analysis
- RNA analysis
- Molecular weight determination
- PCR product verification
- Restriction digest analysis
