Description
High Fidelity PCR Master Mix HFL, 2X is a ready-to-use, pre-optimized PCR master mix formulated with modified thermostable DNA polymerases with 3' to 5' proofreading activity. It produces blunt-ended PCR products with significantly reduced error rates compared to standard Taq, making it a great choice for when sequence accuracy matters: cloning, sequencing template preparation, site-directed mutagenesis, and long-range amplification.
Built-in blue and yellow tracking dyes allow direct gel loading. No separate loading buffer required.
Technical Specifications:
- Format: 2X concentrated master mix
- End modification: Blunt-ended products
- Tracking dyes included: Blue and yellow indicators
- Direct gel loading capability
- Pre-optimized buffer composition
Master Mix Components:
- Modified thermostable DNA polymerases
- PCR reaction buffer
- dNTPs
- Gel tracking dyes
- Stabilizers and enhancers
Research Applications:
- Long-range PCR amplification
- High-fidelity DNA synthesis
- Molecular cloning projects
- Promoter analysis studies
- RACE protocols
- DNA sequencing templates
- GC-rich template amplification
Protocol Features:
- Simplified reaction setup
- Requires only primers and template
- Visual monitoring of electrophoresis
- Compatible with standard thermal cyclers
- Room temperature reaction assembly
Keywords: high-fidelity PCR, long-range PCR, molecular biology reagents, DNA amplification, proofreading PCR mix
