Description
HotStart PCR 2x Master Mix, Red is a ready-to-use, 2X PCR master mix combining chemical hot start activation with long-range amplification capability and a built-in red tracking dye for direct gel loading. No separate loading buffer. No room-temperature polymerase activity during setup. Just clean, specific amplification up to 7kb.
The chemically modified HotStart Taq is inactive at room temperature and activates fully with a 3-5 minute 95°C denaturation step, eliminating non-specific amplification and primer-dimer formation without adding complexity to your cycling protocol.
Technical Specifications:
- Format: 2X concentrated
- Enzyme: HotStart Taq DNA Polymerase
- Activation: 3-5 minutes at 95°C
- Amplification range: Up to 7 kb
- Product ends: Blunt and 3' A-overhang
- Visual indicator: Red dye included
Master Mix Properties:
- Chemical hot-start activation
- Pre-optimized buffer system
- Direct gel loading capability
- Room temperature setup
- Standard PCR cycling conditions
Research Applications:
- High-sensitivity PCR
- High-throughput PCR
- Routine PCR procedures
- Colony PCR screening
- Standard DNA amplification
- PCR cloning projects
Protocol Features:
- Minimized non-specific amplification
- Reduced primer-dimer formation
- Visual reaction monitoring
- Simplified reaction setup
- Template and primer addition only
