Description
HotStart Taq DNA Polymerase is a chemically modified Taq DNA polymerase that remains inactive at room temperature and requires a brief 94°C activation step to begin synthesis. By eliminating polymerase activity during reaction setup, it dramatically reduces primer-dimer formation and non-specific amplification, giving you cleaner results without changing your cycling protocol.
Technical Specifications:
- Activation temperature: 94°C
- Template-dependent polymerization
- Terminal transferase activity: 3' A overhang
- Chemical modification for heat activation
- Storage stable at -20°C
Enzyme Properties:
- Heat-activated DNA synthesis
- Temperature-dependent activity
- 5'→3' polymerase activity
- Compatible with standard PCR conditions
- Inactive at room temperature
Research Applications:
- Genomic DNA amplification
- cDNA target amplification
- Low copy number templates
- GC-rich template amplification
- Multiplex PCR protocols
- Standard PCR procedures
Protocol Benefits:
- Room temperature reaction setup
- Reduced primer-dimer formation
- Minimized non-specific amplification
- Standard PCR cycling conditions
- Compatible with various template types
