Description
Proteinase K Powder (DB0452-1) is a recombinant, lyophilized serine protease produced through recombinant expression for consistent, animal-component-free performance in nucleic acid preparation protocols. Certified DNase/RNase-free, it is suitable for DNA and RNA isolation, PCR template preparation, and any workflow where protein removal and nuclease inactivation are critical.
Technical Specifications:
- Form: Lyophilized powder
- Recommended working concentration: 50-200 µg/ml
- DNase/RNase: Not detected
- Size: 100 mg
- Storage: -20°C
Enzyme Properties:
- Active with or without SDS
- Compatible with urea and EDTA
- Functional across various metal ions
- Activity enhanced by denaturing agents
- Stabilized by Ca²⁺ ions
Research Applications:
- Nucleic acid preparation
- DNA isolation protocols
- RNA purification
- PCR template preparation
- RT-PCR sample processing
- Protein degradation studies
Working Conditions:
- Broad pH range compatibility
- Wide temperature range tolerance
- Active in various buffer conditions
- Functions in presence of common denaturants
