Description
Tsg DNA Polymerase is a thermostable DNA polymerase isolated from Thermus sp., engineered for reliable performance in standard PCR and primer extension applications. The 500U format is the right choice for high-throughput labs, multi-project workflows, or any setting where consistent enzyme availability matters.
Technical Specifications:
- Thermal stability: 3-hour half-life at 95°C
- Error rate: 15x10^6 during DNA synthesis
- Terminal transferase activity: Adds single 3' A overhang
- Unit definition: One unit incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C
- Available in 500U size
- Compatible with standard PCR protocols
Enzyme Properties:
- Isolated from Thermus sp.
- Thermostable at PCR temperatures
- Template-dependent DNA synthesis
- 3' A-overhang generation
- Active across standard PCR conditions
Research Applications:
- Standard PCR protocols
- High-throughput PCR procedures
- Molecular cloning projects
- DNA amplification studies
- TA cloning applications
