Pfu DNA Polymerase, 1000U, high-fidelity proofreading thermostable polymerase from Pyrococcus furiosus, blunt-end PCR products for cloning and mutagenesis

Pfu DNA Polymerase

D151
1000U
$245.00
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Description

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Pfu DNA Polymerase is a high-fidelity, thermostable DNA polymerase originally isolated from Pyrococcus furiosus, a hyperthermophilic archaeon, and expressed recombinantly in E. coli. Its defining feature is a highly active 3' to 5' exonuclease proofreading activity that corrects misincorporated bases during synthesis, producing blunt-ended PCR products with significantly lower error rates than standard Taq polymerase.

For applications where sequence accuracy is critical (cloning, mutagenesis, sequencing template preparation), Pfu gives you the confidence to trust your results.



Enzyme Properties:
- Template-dependent 5'→3' DNA polymerization
- 3'→5' exonuclease proofreading activity
- No 5'→3' exonuclease activity
- Generates blunt-ended PCR products
- Thermostable at PCR temperatures

Technical Specifications:
- Source organism: Pyrococcus furiosus
- Expression system: E. coli
- Proofreading capability: Yes
- End modification: Blunt ends
- Temperature range: Compatible with standard PCR conditions

Research Applications:
- High-fidelity DNA amplification
- Cloning applications
- Site-directed mutagenesis
- Genomic DNA analysis
- Gene expression studies
- Molecular typing protocols

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