Description
Pfu 2X PCR Master Mix brings the high-fidelity proofreading performance of Pfu DNA Polymerase into a convenient, ready-to-use 2X format. All components are pre-optimized -- just add template and primers. Built-in blue and yellow tracking dyes allow direct gel loading with visual monitoring of electrophoresis migration across fragment size ranges.
Technical Specifications:
- Format: 2X concentrated
- Enzyme: Pfu DNA polymerase
- End modification: Blunt ends
- Tracking dyes: Blue and yellow indicators
- Direct gel loading capability
Master Mix Components:
- Pfu DNA polymerase
- Optimized reaction buffer
- dNTPs
- Tracking dyes
- Stabilizers
Research Applications:
- High-fidelity DNA amplification
- Molecular cloning protocols
- Site-directed mutagenesis
- Gene expression studies
- DNA library construction
- Template generation for sequencing
Protocol Features:
- Simplified reaction setup
- Template and primer addition only
- Visual tracking during setup
- Direct gel loading capability
- Room temperature assembly
