Description
Conquest PCR Master Mix-4 is a ready-to-use, 2X PCR master mix formulated with an enhanced processivity buffer for reliable long-range PCR amplification. Standard PCR master mixes lose efficiency on large targets: polymerase stalling, incomplete extension, and low yield become common problems above 3-5kb. Mix-4 is specifically optimized to sustain consistent amplification across extended fragment lengths.
Direct gel loading is built in. No separate loading buffer required.
Technical Specifications:
- Format: 2X concentrated
- Direct gel loading capability
- Enhanced processivity buffer
- Integrated loading dye
- Ready-to-use formulation
Master Mix Properties:
- Long-range amplification
- High-fidelity synthesis
- Room temperature setup
- Standard thermal cycling
- Direct electrophoresis compatibility
Research Applications:
- Long fragment amplification
- Complex genomic regions
- Template-challenging PCR
- Large product synthesis
- Extended fragment analysis
- High-fidelity applications
Protocol Features:
- Simplified reaction setup
- Visual tracking during electrophoresis
- No separate loading buffer required
- Standard laboratory equipment compatible
- Optimized for longer products
