Description
Conquest PCR Master Mix Optimizing Pack is a PCR troubleshooting and optimization tool containing four distinct 2X master mixes, each formulated for a different template challenge. Run them in parallel on your target with your primers, compare the results, and scale up with the mix that works -- all in a single experiment.
Getting four optimized formulations for less than the cost of a single full-size mix makes this one of the most cost-effective ways to establish or rescue a difficult PCR protocol.
Pack Contents:
- Mix-1: Standard genomic amplification
- Mix-2: GC-rich template optimization
- Mix-3: Inhibitor-resistant applications
- Mix-4: Long-range amplification
- All mixes include direct gel loading capability
Optimization Features:
- Systematic PCR condition screening
- Template-specific optimization
- Multiple buffer compositions
- Direct gel loading capability
- Pre-formulated solutions
Research Applications:
- Genotyping protocols
- Genomic DNA amplification
- Complex template PCR
- High GC content templates
- Crude sample processing
- Long fragment synthesis
Protocol Benefits:
- Parallel reaction optimization
- Single-experiment screening
- Direct result comparison
- Simplified workflow
- Reproducible conditions
Selection Process:
1. Run parallel reactions with all mixes
2. Compare amplification results
3. Select optimal mix for template
4. Scale up with chosen formulation
