Description
DNA SafeStain Plus is an advanced fluorescent nucleic acid staining reagent for visualizing DNA and RNA in both agarose and polyacrylamide gels. The "Plus" advantage: broader imaging system compatibility, including blue-light transilluminators and 488nm laser scanners -- making it the right choice for labs that have moved beyond traditional UV-only documentation.
Technical Specifications:
- Concentration: 10,000X stock solution
- Excitation peaks: 290nm and 490nm
- Emission wavelength: 530nm
- Dilution ratios:
• 1:10,000 for pre-cast staining
• 1:5,000 for post-staining
- Compatible with standard imaging systems
Detection Capabilities:
- Double-stranded DNA
- Single-stranded DNA
- RNA molecules
- Agarose gel compatible
- Polyacrylamide gel compatible
Imaging Compatibility:
- UV transilluminators
- Blue-light transilluminators
- 488nm laser scanners
- Gel documentation systems
- Visible light excitation systems
Protocol Features:
- Pre-cast gel staining option
- Post-staining capability
- Running buffer addition option
- Room temperature handling
- Multiple staining methods
Downstream Applications:
- DNA gel extraction
- Molecular cloning
- Fragment purification
- St
