Description
dNTP Set is a complete set of purified deoxynucleotide triphosphates -- dATP, dCTP, dGTP, and dTTP -- formulated at 100mM each for use across a wide range of nucleic acid synthesis applications. Each lot is rigorously quality-tested to ensure nuclease-free, phosphatase-free performance you can rely on from reaction to reaction.
Technical Specifications:
- Chemical purity: ≥99% triphosphate by HPLC
- Concentration: 100mM each nucleotide
- Volume: 400µl each nucleotide
- Amount: 40µmol each nucleotide
- Formulation: In sterile, double-distilled water
Quality Control Testing:
- Nuclease-free certification
- Phosphatase-free certification
- Sanger sequencing verification
- Klenow fragment activity testing
- Thermostable polymerase compatibility
- Spectrophotometric concentration verification
Research Applications:
- Standard PCR protocols
- RT-PCR procedures
- Long-range PCR
- Real-time PCR
- Reverse transcription
- Site-directed mutagenesis
- DNA sequencing
- Primer extension
Storage and Handling:
- Store at -20°C
- Dilute with sterile, double-distilled water
- Avoid repeated freeze-thaw cycles
