Description
Proteinase K Solution is a ready-to-use, broad-spectrum serine protease formulated for molecular biology applications. Most commonly used in DNA and RNA isolation protocols to digest contaminating proteins, inactivate nucleases, and improve nucleic acid yield and purity -- Proteinase K is active across a wide pH range and tolerates the buffer conditions and denaturing agents typically used in extraction workflows.
Technical Specifications:
- Format: Ready-to-use solution
- Volume: 3 ml
- pH range: 4.0-12.5
- Optimal pH: 6.5-9.5
- Temperature optimum: 65°C
- Activity ratio: 12:1 (65°C vs 25°C)
Enzyme Properties:
- Resistant to metal ions
- Resistant to EDTA
- Resistant to sulfhydryl reagents
- Resistant to trypsin inhibitors
- Resistant to chymotrypsin inhibitors
- Enhanced by SDS and urea
Working Parameters:
- Recommended temperature: 55°C
- Maintains activity after partial autolysis
- Stable in various buffer conditions
- Compatible with denaturing agents
- Active against native proteins
Research Applications:
- Nucleic acid preparation
- Protein degradation studies
- Sample preparation protocols
- DNA/RNA isolation
- Tissue dissociation
